ABSTRACT
Estas breves páginas no aspiran a entregar una revisión exhaustiva de lo que son los relojes circadianos, o enumerar los aportes científicos de estos investigadores. Por el contrario, este artículo solo pretende despertar en el lector la curiosidad de revisar literatura especializada e informarse más del tema. Razones hay de sobra.(AU)
These short pages do not aspire to deliver a comprehensive review of what circadian clocks are, or enumerate the scientific contributions of these researchers. On the contrary, this article only intends to awaken in the reader the curiosity to review literature specialized and learn more about the subject. There are plenty of reasons.(AU)
Subject(s)
Humans , Male , Female , Circadian Rhythm , Nobel PrizeABSTRACT
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes.